Scientific Sessions 2024  /  Hyping Up Heart Transplant  /  Race and Ethnic Differences in Donor-Derived Cell-Free DNA and Gene Expression Profiling in Heart Transplant Recipients in a Negative Biopsy Cohort
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American Heart Association

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Final ID: MDP103

Race and Ethnic Differences in Donor-Derived Cell-Free DNA and Gene Expression Profiling in Heart Transplant Recipients in a Negative Biopsy Cohort

Abstract Body (Do not enter title and authors here): Introduction: The Donor-Derived Cell-Free DNA-Outcomes AlloMap Registry (D-OAR) study has highlighted the significant capability of dd-cfDNA, showcasing a 97% negative predictive value (NPV) for identifying both acute cellular rejection (ACR) and antibody-mediated rejection (AMR) at a dd-cfDNA threshold of 0.20%. This development has greatly enhanced the range of biomarkers available to clinicians, confirming dd-cfDNA as a reliable clinical biomarker for monitoring heart transplant (HT) recipients. Short and long-term outcomes for African American HT recipients are worse. Given these findings, our study aims to investigate baseline differences in the levels and positivity rates of dd-cfDNA stratified by race in a negative biopsy cohort.
Methods: We conducted a single-center retrospective review of all HT recipients from 2019 to 2023 with at least one valid combined dd-cfDNA sample drawn between April 2019 and July 2023. Multiorgan transplant recipients and those without a paired biopsy result were excluded. A positive biopsy result was defined as >= ISHLT grade 1R/1B or pAMR>0. Dd-cfDNA (AlloSure, AS) result was considered positive if ≥0.12%, and as a sub-analysis using a threshold ≥0.20%. A positive peripheral GEP (pGEP, AlloMap, AM) test was defined as >30 in the first five months post-transplant and >34 afterward. Samples associated with a positive biopsy result were excluded. We further stratified our cohort based on race.
Results: Of 643 paired dd-cfDNA/GEP samples from 227 patients, the median age was 55 years, and 27% were female. The cohort was stratified into four categories: 53% were white, 18.8% were AA, 20.4% were Hispanic/Latino, and 7.8% were of other races. We observed no baseline differences in the positive dd-cfDNA levels (p=0.39) and positivity rates of dd-cfDNA at a threshold of 0.12% and 0.20% (p=0.08, p=0.06) or GEP positivity rates (p=0.52) between the 4 race categories in our surveillance cohort (Figure 1). Further comparison of patients treated for rejection based on non-invasive monitoring did not reveal a difference.
Conclusions: In our surveillance cohort, dd-cfDNA levels and positivity rates were not different between AA and white HT recipients. Further prospective randomized trials are needed to elucidate potential race-related differences in dd-cfDNA levels.
  • Oren, Daniel  ( NYP-BMH, Weill Cornell Medicine , New York , New York , United States )
  • Moeller, Cathrine  ( Columbia University , New York , New York , United States )
  • Fernandez Valledor, Andrea  ( Columbia University , New York , New York , United States )
  • Rahman, Salwa  ( Columbia university medical center , New York , New York , United States )
  • Rubinstein, Gal  ( Columbia University , New York , New York , United States )
  • Baranowska, Julia  ( Columbia University , New York , New York , United States )
  • Lotan, Dor  ( Columbia University Medical Center , New York City , New York , United States )
  • Defilippis, Ersilia  ( Columbia University Medical Center , New York , New York , United States )
  • Fried, Justin  ( Columbia University Medical Center , New York , New York , United States )
  • Yunis, Adil  ( Columbia University Medical Center , New York City , New York , United States )
  • Raikhelkar, Jayant  ( Columbia university medical center , New York , New York , United States )
  • Clerkin, Kevin  ( Columbia university medical center , New York , New York , United States )
  • Peterson, Stephen J  ( NYP-BMH, Weill Cornell Medicine , New York , New York , United States )
  • Parikh, Manish  ( NYP-BMH, Weill Cornell Medicine , New York , New York , United States )
  • Latif, Farhana  ( Columbia Univerisity Medical Center , New York , New York , United States )
  • Sayer, Gabriel  ( Columbia University Medical Center , New York , New York , United States )
  • Uriel, Nir  ( Columbia University Medical Center , New York City , New York , United States )
    • Author Disclosures:
    Daniel Oren: DO NOT have relevant financial relationships | Adil Yunis: DO NOT have relevant financial relationships | Jayant Raikhelkar: No Answer | Kevin Clerkin: No Answer | Stephen J Peterson: No Answer | Manish Parikh: No Answer | Farhana Latif: DO NOT have relevant financial relationships | Gabriel Sayer: DO have relevant financial relationships ; Consultant:Abbott:Active (exists now) | Nir Uriel: DO have relevant financial relationships ; Research Funding (PI or named investigator):Abbott:Active (exists now) ; Research Funding (PI or named investigator):Fire 1:Active (exists now) ; Research Funding (PI or named investigator):Abiomed:Active (exists now) | Cathrine Moeller: No Answer | Andrea Fernandez Valledor: DO NOT have relevant financial relationships | Salwa Rahman: DO NOT have relevant financial relationships | Gal Rubinstein: No Answer | Julia Baranowska: DO NOT have relevant financial relationships | Dor Lotan: DO have relevant financial relationships ; Speaker:kiniksa:Active (exists now) ; Consultant:viz ai:Past (completed) | Ersilia DeFilippis: DO have relevant financial relationships ; Speaker:Astrazeneca:Active (exists now) ; Other (please indicate in the box next to the company name):Abiomed - member of clinical trial committee:Active (exists now) | Justin Fried: DO NOT have relevant financial relationships
Meeting Info:

Scientific Sessions 2024

2024

Chicago, Illinois

Session Info:

Hyping Up Heart Transplant

Saturday, 11/16/2024 , 09:30AM - 10:55AM

Moderated Digital Poster Session

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