Abstract Body: Non-invasive transcranial direct current stimulation (tDCS) can modulate activity of targeted brain regions. Whether tDCS can reliably and repeatedly modulate intrinsic connectivity of entire brain networks is unclear. We used concurrent tDCS-MRI to investigate the effect of high dose anodal tDCS (4mA) on resting state connectivity between cortical regions within the Arcuate Fasciculus (AF) network, which spans the temporal, parietal, and frontal lobes and is connected via a structural backbone, the Arcuate Fasciculus (AF) white matter tract. The Arcuate Fasciculus Network plays an important role in the auditory-motor feedforward and feedback control of vocalizations and speech-language functions. The degree of injury to the Acuate Fasciculus Network on the dominant hemisphere is related to speech motor/language impairment after stroke. A rudimentary AF-network typically exists on the non-dominant hemisphere that might be enhanced and fostered to show plastic changes after stroke. We tested this approach with non-invasive high dose electrical stimulation. Effects of high-dose tDCS (4mA) delivered via a single electrode placed over one of the AF nodes (single electrode stimulation, SE-S) was compared to the same dose split between multiple electrodes placed over AF-network nodes (multielectrode network stimulation, ME-NETS). While both SE-S and ME-NETS significantly modulated connectivity between AF network nodes (increasing connectivity during stimulation epochs), ME-NETS had a significantly larger and more reliable effect than SE-S. Moreover, comparison with a control network, the Inferior Longitudinal Fasciculus (ILF) network on the same hemisphere targeted by tDCS suggested that the effect of ME-NETS on connectivity was specific to the targeted AF-network. This finding was further supported by the results of a seed-to-voxel analysis wherein we found ME-NETS primarily modulated connectivity between AF-network nodes, meaning cortical regions such as the Inferior Frontal Gyrus, the Supramarginal Gyrus, and the posterior superior temporal gyrus, which constitute the nodal regions of the AF network. Finally, an exploratory analysis looking at dynamic connectivity using sliding window correlation found strong and immediate modulation of connectivity during three stimulation epochs intermingled with no-stimulation epochs within the same imaging session suggesting that the intrinsic connectivity between these regions can reliably and repeatedly be modulated.