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American Heart Association

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Final ID: P-300

Modulation of Osmolyte Levels by ACE Inhibitors and SGLT-2 Inhibitors in Normotensive Rats

Abstract Body: Hypertension is associated with disturbances in the water-electrolyte balance, leading to changes in osmotic pressure. Osmolytes, such as taurine among other compounds, play a critical role in maintaining cellular integrity under osmotic stress.
This study evaluated the effects of ACE inhibitors (ACE-I) and SGLT-2 inhibitors (SGLT2i) on osmolyte homeostasis. Twelve-week-old male Sprague-Dawley (SD, n = 38) rats were divided into five groups: control (water), low and high doses of canagliflozin, and low and high doses of captopril. Metabolic studies were conducted in metabolic cages. Using liquid chromatography coupled with mass spectrometry (LC-MS), we measured osmolyte concentrations in blood, urine, kidney, liver and heart tissues.
The obtained results demonstrated significant increases in liver taurine concentrations (13.34 ± 1.39 vs. 10.49 ± 1.45, p=0.029) and urinary taurine excretion (25.08 ± 2.81 vs. 16.54 ± 3.77, p=0.001) in the high-dose canagliflozin group compared to controls. Both treatments enhanced the total urinary excretion of L-alanine, β-alanine, choline, glycine, taurine, and carnitine. Additionally, canagliflozin increased the excretion of betaine and glycerophosphorylcholine (GPC). A reduction in serum β-alanine was noted in the captopril-treated rats.
These findings highlight the novel effects of SGLT-2 inhibitors and ACE-I on osmolyte levels in normotensive rats, suggesting potential mechanisms contributing to their cardioprotective effects. Further research is required to elucidate the clinical significance of osmolyte changes in enhancing the protective effects of these drug classes.
  • Rybka, Mateusz  ( Medical University of Warsaw , Warszawa , Poland )
  • Mazurek, Lukasz  ( Medical University of Warsaw , Warszawa , Poland )
  • Szudzik, Mateusz  ( Medical University of Warsaw , Warszawa , Poland )
  • Samborowska, Emilia  ( Polish Academy of Sciences , Warsaw , Poland )
  • Ufnal, Marcin  ( Medical University of Warsaw , Warszawa , Poland )
  • Konop, Marek  ( Medical University of Warsaw , Warszawa , Poland )
  • Author Disclosures:
    Mateusz Rybka: No Answer | Lukasz Mazurek: No Answer | Mateusz Szudzik: No Answer | Emilia Samborowska: No Answer | Marcin Ufnal: DO have relevant financial relationships ; Employee:AstraZeneca:Active (exists now) | Marek Konop: DO NOT have relevant financial relationships
Meeting Info:
Session Info:

Poster Session 2

Friday, 09/06/2024 , 09:00AM - 10:30AM

Poster Session

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