Logo

American Heart Association

  27
  0


Final ID: MDP814

Analysis of Polarization Changes in Ascending Aortic Endothelial Cells Induced by Aortic Valve Regurgitation

Abstract Body (Do not enter title and authors here):
Background: Fragility of the ascending aortic wall has been reported in patients with aortic regurgitation (AR), thought to be affected by turbulence in the ascending aorta. However, the mechanism behind this effect is unclear. We hypothesized that turbulence in the ascending aorta of AR patients causes changes in the polarization of endothelial cells. This study aimed to clarify that the polarization of endothelial cells in the ascending aortic wall is altered in AR patients.
Methods: Twenty patients who underwent ascending aortic replacement at our institution from October 2022 to July 2023 were included: ten with thoracic aortic aneurysms (TAA) and ten with TAA and AR. The polarization of endothelial cells in the greater curvature of the ascending aortic wall collected during surgery was evaluated by measuring the ratio of the long axis to the short axis, the angle between the long axis and the blood flow axis, and the alignment of the Golgi apparatus and nucleus parallel to the blood flow.
Results: There were no significant differences in patient background between the AR and non-AR groups. Additionally, there was no significant difference in the diameter of the ascending aorta between the two groups (AR vs. non-AR, 47.7±3.8 mm vs. 44.5±2.2 mm, p=0.479). The long axis to short axis ratio (aspect ratio) of endothelial cells in the ascending aorta was significantly smaller in the AR group than in the non-AR group. The angle (θ) between the long axis of the endothelial cells and the blood flow axis was significantly smaller in the AR group than in the non-AR group. The ratio of Golgi apparatus and nucleus alignment parallel to the blood flow was significantly lower in the AR group than in the non-AR group. Degeneration of the aortic tunica media was also observed in the AR group compared to the non-AR group.
Conclusion: The results of this study suggest that the presence of AR alters the polarization of endothelial cells in the ascending aortic wall.
  • Tamagawa, Yuki  ( Osaka University , Osaka , Japan )
  • Miyagawa, Shigeru  ( Osaka University , Suita , Japan )
  • Kawamura, Takuji  ( OSAKA UNIVERSITY , Osaka , Japan )
  • Harada, Akima  ( Osaka University , Osaka , Japan )
  • Miyake, Keisuke  ( OSAKA UNIVERSITY , Suita , Japan )
  • Kawamura, Ai  ( Osaka University , Suita-city, Osaka , Japan )
  • Kido, Takashi  ( Osaka University , Osaka , Japan )
  • Yamashita, Kizuku  ( Osaka University , Suita , Japan )
  • Shimamura, Kazuo  ( OSAKA UNIVERSITY , Osaka , Japan )
  • Saito, Shunsuke  ( Osaka University , Suita, Osaka , Japan )
  • Author Disclosures:
    Yuki Tamagawa: DO NOT have relevant financial relationships | Shigeru Miyagawa: DO NOT have relevant financial relationships | Takuji Kawamura: No Answer | Akima Harada: DO NOT have relevant financial relationships | Keisuke Miyake: DO NOT have relevant financial relationships | Ai Kawamura: DO NOT have relevant financial relationships | Takashi Kido: No Answer | Kizuku Yamashita: DO NOT have relevant financial relationships | Kazuo Shimamura: DO NOT have relevant financial relationships | Shunsuke Saito: DO NOT have relevant financial relationships
Meeting Info:

Scientific Sessions 2024

2024

Chicago, Illinois

Session Info:

Emerging Mechanisms of Cardiovascular Diseases

Sunday, 11/17/2024 , 11:10AM - 12:35PM

Moderated Digital Poster Session

More abstracts on this topic:
Arterial Stiffness in Obese Normotensive Children: The First Cardiovascular Risk Biomarker?

Duarte Mariano, Aquieri Analia, Rivas Silvia, Donth Leger Gimena, Pardo Valeria, Coyle Javier, Curia Andrea, Gonzalez Ballerga Esteban

Machine learning identifies clinically distinct phenotypes in patients with aortic regurgitation.

Deb Brototo, Scott Christopher, Michelena Hector, Pislaru Sorin, Nkomo Vuyisile, Kane Garvan, Crestanello Juan, Pellikka Patricia, Anand Vidhu

More abstracts from these authors:
Safety and Therapeutic Potential of Allogeneic Adipose-Derived Stem Cell Spray Transplantation in Ischemic Cardiomyopathy: A Phase I Clinical Trial

Kawamura Takuji, Yoshioka Daisuke, Kawamura Ai, Misumi Yusuke, Taguchi Takura, Saito Shunsuke, Yamauchi Takashi, Miyagawa Shigeru

Clinical Evidence of Immune Response to Transplanted Allogeneic iPS Cell-Derived Cardiomyocytes

Kawamura Takuji, Ito Emiko, Takeda Maki, Yoshioka Daisuke, Kawamura Ai, Misumi Yusuke, Saito Shunsuke, Yamauchi Takashi, Miyagawa Shigeru

You have to be authorized to contact abstract author. Please, Login
Not Available