Abstract Body: BACKGROUND Comprehensive Stroke Center assigned annual education using self-learning [SL] via an online learning system [OLS] with post-test upon completion. Education for all staff included BE FAST to remember stroke symptoms and immediate actions to take when recognized for in-hospital stroke alert activation [IH-SA-A]. “BE FAST Fridays” [BFF] were implemented May 2024 and voluntary in-person, one-on-one education [1:1E] was conducted every Friday to assess current knowledge, review BE FAST and actions to take for IH-SA-A. The teach-back method was used to assess overall understanding. Flyers and day-of-event overhead announcements were shared for staff awareness. Event was held in the hospital front lobby for visibility. Breakfast and stroke t-shirts were provided after completing 1:1E. Designated “BE FAST Office Hours” [BFOH] were implemented June 2024 to allow for ongoing 1:1E. PURPOSE Evaluate the impact of SL via an OLS and 1:1E conducted through BFF and BFOH on IH-SA-A. METHOD Retrospective review of 1,202 education records in 2024 was conducted to determine total 1:1E participation. Participants were grouped as re-education [1:1E after SL] and initial education/new staff [1:1E only] for analysis. Retrospective review of 54 IH-SA-A was conducted to determine those that occurred before 1:1E [PRE] Jan 2022-April 2024 and after 1:1E [POST] May-July 2024. POST IH-SA-A were evaluated for 1) positive brain imaging [PBI]; 2) transfer to higher level of care [THLC]; 3) thrombolytic administration [TA]; and 4) mechanical thrombectomy [MT]. All groups were divided into hospital departments [HD] for analysis. RESULTS Total participants 655, included 76% 1:1E after SL and 24% 1:1E only. Highest number of participants and 1:1E after SL were PACU/Adult Units [P/A], Women’s Services [WS], and Other Staff [OS]. Highest number of 1:1E only were P/A and OS. IH-SA-A increased from monthly average of 1.57 PRE to 3.33 POST with increases in all HD. Largest increase was in Stroke Unit and P/A. WS had 1 IH-SA-A POST after having 0 PRE. Outcomes of POST IH-SA-A included 50% PBI, 63% THLC, and 10% MT. CONCLUSION Education using 1:1E format provided enhanced individualized experiences that resulted in increased BE FAST recognition leading to increased IH-SA-A. While SL provides flexibility for completion and ease of tracking, staff are more likely to benefit from 1:1E that encourages active listening/discussion, allows for questions, and provides targeted feedback.