Abstract Body: Introduction: Parental exposure to particulate matter (PM) represents a critical window influencing health outcomes in offspring. While the effects on male progeny are better characterized, whether a similar phenotype occurs in females remains under explored. Therefore, we investigated disease inheritance in female offspring and hypothesized a phenotype like that previously shown in males. Methods: Parent FVB sire and dam mice were exposed to either filtered air (FA) or PM with an aerodynamic diameter <2.5 in (PM_2.5) for 6 h/day (avg of 36.0 μg/m³), 5 d/week for 3 months. Following exposure, mice were bred to produce female FA_sFA_d(both sire and dam exposure to FA) and PM_sPM_d (both sire and dam exposed to PM). Cardiovascular and metabolic health were measured at 18, 30, and 46 weeks via echocardiography, tail cuff plethysmography, and glucose tolerance testing (GTT). Lung mechanics (Flexivent) and cardiac pressure-volume (PV) loops were performed following 46-week measurements. Data were analyzed using mixed model analyses with comparisons between exposure and time via Sidak’s test. Significance was set at p<0.05. Results: Female PM_sPM_d left ventricle diastolic volume and diameter were elevated at 18 (both p=0.08) and 46 weeks (p=0.03). Ejection fraction (EF) and fractional shortening (FS) revealed transient deficits at 30 weeks (both p<0.01) which may have been influenced by reduced n at 46 weeks (n=3). Transient reductions at 18 weeks were also detected in blood pressures (all p<0.05) and GTT (AUC p=0.01) in PM_sPM_d. PV loop analysis revealed PM_sPM_d operating pressures did not differ (P_ed and P_es, both p>0.05) despite an increased end-diastolic volume (p<0.01) and supported by reduced contractility (Ees) compared to FA_sFA_d (p=0.01). Conclusion: By limiting PM_2.5 exposure to sire and dam prior to conception, an altered cardiac and metabolic phenotype was present in female offspring. Together with previous evidence in males, these data suggest potential for an epigenetically regulated pattern of disease that appears independent of sex.